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AAMToolbox: A Shape and Appearance Modelling Toolbox for Matlab User Guide Dr. A. I. Hanna 2007 aih@cmp.uea.ac.uk Dr. A. I. Hanna Page 1 16/11/2007
Table of Contents Installing the software................................................................................................................ 4 Getting Started........................................................................................................................... 5 Overview of Toolbox.................................................................................................................. 6 Image Processing Tool.............................................................................................................. 8 Selecting images ................................................................................................................... 8 Resolution ............................................................................................................................. 8 Crop and Select..................................................................................................................... 8 Template Editor Tool ................................................................................................................. 9 Importing Templates.............................................................................................................. 9 New Templates ................................................................................................................... 10 Choosing Primary Points..................................................................................................... 10 Point Connectivity (Drawing Lines or edges) ...................................................................... 10 Saving the Point Model Template ....................................................................................... 11 Global Point Model Editing.................................................................................................. 11 New Project Tool ..................................................................................................................... 12 Making Augmented Projects.................................................................................................... 13 Importing Group Means........................................................................................................... 15 Choose Template Tool ............................................................................................................ 15 Point Model Editor Tool ........................................................................................................... 15 Moving Points...................................................................................................................... 16 Smoothing Points ................................................................................................................ 16 Other functions .................................................................................................................... 17 Automatic Placement Tool....................................................................................................... 17 Segmentation Fitting ........................................................................................................... 17 Subset Fitting ...................................................................................................................... 19 Likelihood Fitting ................................................................................................................. 19 Set Picker Tool ........................................................................................................................ 19 Modelling Subsets in Context.............................................................................................. 21 Select Images...................................................................................................................... 21 View Stats Model (PDM Walk) Tool ........................................................................................ 22 Shape Space Tool ................................................................................................................... 23 Creating Figures for Papers .................................................................................................... 28 Updating existing templates and point models........................................................................ 32 Conversion between old and new point model templates......... Error! Bookmark not defined. Comments ............................................................................................................................... 33 Allometry model – the Mathematics ........................................................................................ 33 Contact Information: ................................................................................................................ 36 Dr. A. I. Hanna Page 2 16/11/2007
Table of Figures Figure 1 Computational Biology Download Page Figure 2 Setting the path Figure 3 Directory structure used by the Shape Model toolbox. Figure 4 First look Figure 5 Image processing toolbox Figure 6 Cropping the image Figure 7 Template Editor before and after loading (or creating) a template Figure 8 Smoothing secondaries Figure 9 Adding edges between points 1 1 1 1 1 Error! Bookmark not defined. 9 1 1 Dr. A. I. Hanna Page 3 16/11/2007
now copy the copied section for (please see Installing the software The active appearance model toolbox (AAMToolbox) can be downloaded from the author’s website, AAMToolbox and click on the icon next to the link shown below (Figure 1). Once you have downloaded the zip file Matlab_files_AIH_JS.zip you can either unzip this using WinZip or if you are using Windows XP, you should be able to access the files directly. Either way you must folders “Matlab_files_AIH” and “Matlab_files_JS” to your specified root “Shape_models” the directory directory hierarchy a more comprehensive explanation of this). Having folders Matlab_files_AIH” and “Matlab_files_JS”, you can start up your version of Matlab (note: this software has currently only been tested on Matlab version 7.3.0 R2006b). At the command prompt in Matlab type (notice here we have used ‘>>’ symbols to show we are at the command line): >>pathtool This will bring up a Matlab graphical user interface (GUI) tool that will allow you to set the path to the previously copied Matlab files, see Figure 2. As indicated in the figure you should now click the button entitled “Add with Subfolders”, this will then prompt you for a directory. Please choose the directory “Matlab_files_AIH” that you copied as explained in the previous paragraph. Click “Add with Subfolders” then save the new path settings and close. Figure 1b Authors Download Site the Figure 1a Computational Biology Download Page the Dr. A. I. Hanna Page 4 16/11/2007
Figure 2 Setting the path To test that the installation was successful please type the following at the Matlab command line prompt: >> AAMToolbox_InstallTest.m If the installation was successful, the user will be presented with a small dialog box like the one shown below. Getting Started At this stage you should be happy that you have downloaded and copied the Matlab files into a suitable location. The directory structure at this point should be something like the one shown in Figure 3 (notice that the Matlab files are in a directory called “Shape_models” that also contains a folder called “Models”): Now you are in a position to start the toolbox, to do this type the following at the Matlab command line prompt: >>AAMToolbox is When opened from a directory that is not a Project the Figure 3 Directory structure used by the Shape Model toolbox. the toolbox Dr. A. I. Hanna Page 5 16/11/2007
Current Template and Sample image axes are empty and the Current Working Project indicates ‘No Project Selected’ It can be seen from Figure 4 that if you are not currently working in a project directory then the system will indicate ‘No Project Selected’. Use the ‘Browse’ option to select a project. Note display showing the AAMToolbox in the current path. Overview of Toolbox Opening the toolbox from a directory that is a Project the Current Template and a Sample image is displayed. The toolbox contains a set of tools that work with a Statistical Model project. There are two ways to access data in a project Figure 4: First look 1. Using the graphical user interface (GUI) called AAMToolbox 2. Programming in Matlab and accessing the data through the SM class library Here we only discuss the GUI. The details of the project and directories are displayed in the Project Details section of the toolbox at the top. Selecting a tool causes the toolbox window to disappear and open a window for the tool. To return to the toolbox, close the tool or click on the ‘back to toolbox icon’. If, for some reason, the windows refuse to close normally they can be forced to close by typing the command ‘CloseAAMtoolbox’. A project is based on a specific set of images. Each project contains 5 folders that the original contain images, processed images, templates, point models and statistical models. You can change or specify the project you are working on using the browse option in the Project Details section. Projects are created using a series of tools in the toolbox. 1. The first tool is New Project and this asks for the name and location of the desired project. The system automatically adds the prefix: ‘PRJ_’. Be careful in choosing the name to ensure that it reflects the images that the project contains. The New Project tool can also be used to combine or augment pre-existing projects. You can also import one project into another. 2. The next stage is to import images into the original and processed images subfolders of the project. This is done manually by copying and pasting the images into the relevant folders. Each jpeg image should have a distinct name (i.e. imagename.jpg). It is useful if the name represents useful information, e.g. experimental details, subject name, date, etc. The Details of the selected model Figure 5: AAMToolbox interface Dr. A. I. Hanna Page 6 16/11/2007
processed images should all have the same size in pixels (a convenient size is 800 pixels wide). To help with processing, you can use the image processing tool. Note that if you want to create an allometric model (one that does not normalise size), you need to untick the scale box in the Procrustes section of the SMG (Statistical Model Generator) tool (see below). 3. You then need to import at least one template into the Templates folder of the project. This can either be done manually by copying previous templates and pasting, or using the template editor tool to browse the list of templates. If you do not have a template you will need to create one using the template editor tool. Templates all have the suffix .temp_dat. (In some cases a supplementary file with the suffix .edit_set will be created.) Point Model Place points defined by the template into the correct positions on every image Stats Model Gen. Generate statistical model from multiple Point Models View Stats Model View the statistical model generated from the Point Models Figure 6 Point models to Statistical models. 4. To start creating models (Point Models for each image and Statistical models derived from multiple images) you need to assign one template from the Templates folder using the choose template tool. This will create folders with the template name in PointModels and StatisticalModels directories (i.e. Pointmodels/template_name). Thus all models based on this template will carry the template_name as an identifier. 5. To define the positions of points in relation to the images there are two options. A. Point models are created according to the template (selected previously, see above). They are usual created manually using the Point Model Editor (PM_editor) tool. Points are moved to the appropriate feature points in the image. A Point Model is created for each image in the Cropped directory. The pm (point model) files are stored subfolder within PointModels directory. (i.e.pointmodels/template_name/imagename.pm). Note that these are not exclusive – you could first use Autoplacement and then check the points manually using PM_editor template-name in the B. For some image shapes (e.g. leaves), you can automatically place points on your images using the Autoplacement tool. When you invoke this tool, it will ask you whether you want to place points through segmentation, warp all points according to a subset of points, or fit using a probabilistic model based on a subset of points. In the segmentation option you will be asked to specify the type of image you want to segment (e.g. petals, leaves etc) and will then create a set of binary images in a processed images/binary subfolder. You then click on a few key points and draft point models are created. 7. When Point Models have been created for each image you need to consider which points to use for your statistical point model. Models associated with each set are stored in separate subfolders of the StatisticalModels folder. By default all points of the template are used to create the statistical point model. This is stored in a subfolder called Set_1 located in StatisticalModels/ template_name. You may also want to select a subset of points. This is done using Point Sets tool that allows you to choose the subset of points to be used. The point sub-set information is stored in a newly created folder called Set_2 (further subsets will be named consecutively) located in StatisticalModels/ template_name. You can also give the selection of points a logical name and this will be displayed in the project details area. 8. To build a statistical model you now use the SMG (Statistical Model Generator) tool. Using this, you first need to choose the images that should be included in the statistical models. By default all images in processed images will be used. This will create a subfolder called ImageList_1 in StatisticalModels/ template_name /Set_1. You may also want to select a subset of images. This is done using a drop-down menu within the SMG tool. This allows Dr. A. I. Hanna Page 7 16/11/2007
you to choose the subset of images to be used and when this is complete, the image set information is stored in a newly created folder called ImageList_2 (further subsets will be named consecutively) located in StatisticalModels/ template_name /Set_1. You can also give the selection of images a logical name and this will be displayed in the project details area. Within SMG you can also generate a combined shape and appearance model. (You can also generate appearance models based on treating each triangle as a patch or using wavelets: to be done). 9. Statistical models can be displayed using the View Stats Model tool. Within this you can A. Examine how each principal component affects shape and appearance B. Make movies of the shape and appearance changes C. Import images to modify the mean appearance D. Evaluate how well an image is created by the model compares to the original image E. Examine the effect of quantising shape and appearance space 10. Statistical models can be viewed in shape space 2 or 3D shape space using the View Shape Space tool. the images from Image Processing Tool Within the Image Processing tool you can perform a number of operations on the images stored in the directory processed images. This tool assumes that you have copied the originals directory and are ready to process them by resizing, cropping and selecting regions to remove background noise. In practice, it is often easier to write your own Matlab image processing tools or even use an image editor such as Photoshop. When you select the Image Processing tool you will be presented with the following window, This tool automatically chooses the images in the processed to be processed. images directory as the ones Figure 5 Image processing toolbox Selecting images To navigate through the images you can either click the “Next” and “Prev” buttons or click on the drop down menu that shows the image filename and skip to any image in the list. Resolution To change the resolution of an image (i.e. reduce or increase the number of pixels per m210- ) you type in the ratio you require (shown at 0.5 in the above figure which will reduce the number of pixels in the image by half) and then you can either apply this ratio to the shown image by clicking on the “Change Resolution” button, or you can apply it to all the images in the processed images directory by clicking on the “Apply To All” button. Crop and Select To crop an image you type in the number of pixels you require for the width and height in the text boxes provided in the ‘Crop Panel’ and then click on the “Crop” button. The mouse cursor will then change to a full crosshair; you are then required to click on the center of the required region. Notice that if you type in a crop size that is greater than the size of the image then it is padded with zeros. Dr. A. I. Hanna Page 8 16/11/2007
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